Faculty of Science

Lee Lab

Dr. Lee's research centers on the use of cell culture models derived from non-mammalian species, specifically fish and economically important invertebrates such as bees, shrimp, and lobsters.

Specifically, Dr. Lee's interest revolves around developing continuous cell lines that can be readily available and used for evaluating effects of nutrients, hormones, drugs, contaminants, toxicants, for applications in animal health, animal husbandry, biotechnology, environmental monitoring, etc.

Current projects funded by the Natural Sciences and Engineering Research Council of Canada (NSERC) include the development of fish cell lines and their use in the study of intracellular parasites like the microsporidia that have become emerging pathogens of concern.

Dr. Lee’s team members have used classical cell-culture methodologies along with proteomics and immunotechniques to understand interactions of host cells with model intracellular and extracellular aquatic pathogens. Recent interests have expanded to cover areas of concern in agriculture such as the study of microsporidians affecting bees, climate change and pesticide effects in non-target species, and evaluation of nutraceuticals, specifically, evaluating blueberry components for fish diets using fish intestinal cells (the latter funded by an NSERC Strategic).

Dr. Lee has adjunct faculty appointments at the University of British Columbia, Zoology Department; the University of Waterloo, Department of Biology; Wilfrid Laurier University, Department of Biology; as well as graduate appointments at the University of Guelph, Biomedical Sciences; and as such has supervised or co-supervised many graduate students.

At UFV, Dr. Lee has supervised several undergraduate and post-graduate students in various research projects and these include:

  • Eric Sung-Jae Cho (Fall 2019) volunteer assistant, general cell culture, quantitative methods
  • Heather Michelle Kelly (Fall 2019) volunteer assistant, general cell culture, quantitative methods
  • Eryn Braley (Summer 2019) Evaluation of common pesticides on fish cell lines (S’19 NSERC USRA)
  • Michael Mong (Summer 2019) Characterizing fish muscle cell lines (co-op UBC)
  • Brenna Hay (Sep’18-Aug’19) Genotoxic effects of neonicotinoids in fish cells (W’19 NSERC-USRA)
  • Arianne Qanberry (Sep’18-May’19) Effect of temperature fluctuations in vitro on fish mitochondria
  • Petra Lee (Sep’18–Oct’19) Fish and shrimp cell culture (part-time technician)
  • Anthony Miyagi (Summers 2018, 2019) Initiation and culture of invertebrate cells: bees and shrimp
  • Taylor Boyd (Winter and Summer 2018) Culture of trout olfactory cells (W’18 NSERC-USRA)
  • Tessa Webb (Summer, Fall 2017) Fish mesenchymal cell culture maintenance and quality control
  • Veronica Kobes (Summer 2017/18) Histology of rainbow trout tissues (S’18 NSERC-USRA)
  • Sergio Navarro (Summer 2017) Primary cell cultures of rainbow trout, routine fish cell line growth
  • Rachael Hyun Bae (summer 2016) Cell culture maintenance, mycoplasma testing
  • Harshraj Sidhu (Winters 2016, 2017) In vitro toxicology (W’16 NSERC USRA), and, Co-cultures of gastrointestinal and immune cells (W’17 NSERC USRA)
  • Kamal Moghrabi (Summer 2015 to 2016) Neonicotinoid effects in fish cells (W’15 NSERC-USRA)
  • Gagandeep Rai (Summer 2015 and 2016) Fish feed evaluation (S’15 & S’16 NSERC-USRA)
  • Gaganjeet Mahil (Winter 2016) Evaluation of blueberry extracts w fish cells (W’16 NSERC-USRA)
  • Vishesh Oberoi (2015) Scratch assays, Blueberry extract effects on fish (W’15 NSERC-USRA)
  • Alyssa Zucchet (Summer 2015) Cell maintenance, lab upkeep
  • Hardeep Saran (Summer 2015) Basic fish cell culture
  • Manjot Birk (Winter 2015) routine fish cell line maintenance
  • Kevin Kobes (2014) Evaluating physicochemical factors on the growth of fish and invertebrate cells
  • Mandeep Kaur Saran (2014) Characterizing cod cells (S’14 NSERC USRA)
  • John Andrew Alexander (2013-14) Quality control of fish and insect cells (S’13,14 NSERC USRA)
  • Fabiola Rojas (2013, 2014) Assistance with cell culture lab maintenance and organization
  • Marcus Cardoso Trento (2013) Genotoxic evaluation of agricultural contaminants

Invited Lectures & Talks

2019

  1. Saving the Ocean Fish: Growing Fish Meat in a Lab. Abbotsford Learning Plus Society, Abbotsford, BC. November 5. [PowerPoint Presentation Slides]
  2. Cellular Agriculture/ Aquaculture: Meat in a lab. Plant & Food Research, Nelson, New Zealand. February 28.

2018

  1. Cellular Agriculture: Pros and Cons of Growing Meat in a Lab. UFV Faculty of Science greenSPEAK Series December 5.
  2. Ecotoxicology & Fish Invitromatics. Toxicology Centre, University of Saskatchewan, Saskatoon, SK. August 29.
  3. Marine Fish Invitromatics. Plant & Food Research, Nelson, New Zealand. April 24.
  4. Development and Use of Various Marine Fish Cell Lines. Universidad Austral de Chile, Valdivia, Chile. March February 20.
  5. Fish Invitromatics. Dean of Science Seminar Series, University of the Fraser Valley, Abbotsfod, BC. February 20.
  6. Invitromatics: The Art & Science of Cell Culture. Comparative Physiology Group, Department of Zoology, UBC. January 22.
  7. KFE-5, a myogenic cell line derived from embryonic mesodermal tissue of killifish, Fundulus heteroclitus. Finless Fish, Berkeley, CA. January 15.

2017

  1. The use of fish intestinal cells for aquaculture feed research. Norwegian Veterinary Institute, Oslo, Norway. September 25.
  2. Role of fish cell lines in fisheries and aquaculture. Norwegian Veterinary Institute, Oslo, Norway. September 25.
  3. Twenty years of research with the trout monocytic cell lines RTS-11, Austral University Chile, Interdisciplinary Centre for Aquaculture Research, January 16.
  4. History, develoment and use of fish cell lines. Collagen forming fish cell lines and their use, Austral University Chile, Biochemistry and Microbiology Insitute, January 19.

2016

  1. Avoiding questionable journals/publishers, Semi-annual meeting of BC Deans of Arts and Science Programs, Kelowna, BC
  2. Fish cell lines as tools in fish health and environmental monitoring, UFV Microlectures, February 24
  3. The Korean gender gap & Moving Forward, International Women’s day, UFV SUB, March 8
  4. Open Access Journals and Predatory Publishers, BC Deans of Arts and Science meeting, Kelowna, BC, April 12

2015

  1. Evaluating effects of neonicotinoid pesticides using fish cell lines. ToxTalks 2015, Simon Fraser University, Burnaby, BC
  2. Survival through one of the top 10 deadliest earthquakes: Peru 1970, Abbotsford learning Plus, Abbotsford Recreation Centre, February 3
  3. Sustainability in the face of a megathrust earthquake, Green UFV talk and Discovery Speaker Series, UFV Geography, March 12
  4. Neonicotinoid pesticides and the Bees, Chilliwack Rotary Club, Chilliwack, BC, April 24
  5. Microsporidians. Intro to Mycology Guest lecture, BIO370, A261, UFV, September 30
  6. Goal 14: Life below water. UFV and the 2030 UN Sustainable Development goals. SUB, October 13
  7. Cascadia & Megathrust Earthquakes, Tapestry for thought presentation, UFV Elder College, Chilliwack, November 4

2014

  1. Space waste monitoring and Sapphire, Cheam Probus (Chilliwack) January 23
  2. Oil sands and fish cell lines. Abbotsford Learning Plus, Abbotsford Recreation Centre, February 4
  3. Nosema and bees, fish and human health. Richmond Bee Club, BC, May 17
  4. Nosema: A story about bees, fish and human health. Maple Ridge Bee Keepers Association, BC, June 23
  5. Non-mammalian cell cultures to the rescue, how they can help apiculturists. Langley Bee Club, Langley, BC, September 2
  6. Sapphire and space junk , Tapestry for thought presentation, UFV Elder College, Chilliwack, November 19

2013

  1. Development and applications of non-mammalian cell lines to biotechnology, aquaculture and apiculture. Physiology group, Dept of Zoology, UBC, January 23
  2. Sustainability in Space and Earth. UFV’s role with Sapphire. Green Speak Series, Centre for Environmental Sustainability, UFV, Abbotsford, BC, February 19
  3. Evaluating Toxicity and remediation strategies in oil sands process-affected waters using fish cell lines. Green Speak Series, Centre for Environmental Sustainability, UFV, Abbotsford, BC, April 2
  4. Potential contributions of non-mammalian cell lines to biotechnological advancements in agriculture and environmental sustainability. Discoveries Speaker Series, Geography Department, UFV, Abbotsford, BC, September 19
  5. Sapphire satellite and sustainability in space. Abbotsford Learning Plus, Abbotsford Recreation Centre, October 1
  6. Sustainability in space and the Sapphire Surveillance Satellite. Cheam Probus (Coast Hotel, Chilliwack) October 15
  7. Microsporidian Research, Guest lecture for Mycology BIO370, A255, UFV, November 4

2012

  1. Scientific Investigation with Fish Cell lines. International Congress for Scientific Investigation. University of Cesar Vallejo, Trujillo, Peru, September 22

Publicatons

2013-2019 (Underlined names are those of my trainees)

  1. Pumputis PG, Dayeh VR, Lee LEJ, Hamilton ME,Viththiyapaskaran S, Bols NC. 2019. Beneficial and detrimental effects of the phytochemical naringenin on rainbow trout intestinal cells. Journal of Functional Foods (accepted Oct 27, 2019, JFF-D-19-02152R1).
  2. Schirmer K, Stadnicka J, Belanger S, Blaha L, Bols N, Dyer S, Embry M, Fischer M, Halder M, Hermens J, Hultman M, Kramer N, Laue H, Lee L, Lillicrap A, Natsch A, Segner H, Tanneberger K, Tollefsen K, Werner I, Witters H, Zupanic A. 2019. Cell-based data to predict the toxicity of chemicals to fish. Commentary on the manuscript by Rodrigues et al., 2019. Environmental Pollution 252:476-482.
  3. Pham PH, Sokeechand BSH, Hamilton ME, Misk E, Jones G, Lee LEJ, Lumsden JS, Bols NC. 2019. VER-155008 induced Hsp70 proteins expression in fish cell cultures while impeding replication of two RNA viruses. Antiviral Research 162: 151-162.
  4. MacLeod MJ, Vo NTK, Mikhaeil MS, Monaghan SR, Alexander JAN, Saran, MK, Lee LEJ. 2018. Development of a Continuous Cell Line from Larval Atlantic Cod (Gadus morhua) and its Use in the Study of the Microsporidian, Loma morhua. J Fish Diseases 41(9): 1359-1372.
  5. Pumputis PG, Dayeh VR, Lee LEJ, Pham PH, Viththiyapaskaran S, Bols NC. 2018. Responses of rainbow trout intestinal epithelial cells to different kinds of nutritional deprivation. Fish Physiol Biochem 44: 1197-1214.
  6. Kim JJ, Hamilton ME, Pham PH, Lee LEJ, Bols NC. 2018. Effect of selenomethionine on cell viability and heat shock protein 70 levels in rainbow trout intestinal epithelial cells at hypo-, normo-, and hyper-thermic temperatures. J Thermal Biol 76: 107-114.
  7. Trento MVC, Cesar PHS, Marcussi S, Lee LEJ. 2018. Genotoxic action of naphthenic acids on the fish macrophage cell line, RTS11. Int J Environ Poll 63: 117- 130.
  8. Bols NC, Pham PH, Dayeh VR, Lee LEJ. 2017. Invitromatics, invitrome and invitroomics: Introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout. In Vitro cell dev Bio Anim. (Ms # IVAN-D-16-00404R1, accepted Feb 6, 2017) http://onlinelibrary.wiley.com/doi/10.1002/etc.3603/epdf
  9. Lillicrap A, Belanger S, Burden N, Du Pasquier D, Embry M, Halder M, Lampi M, Lee LEJ, Norbert-King T, Rattner BA, Schirmer K, Thomas P. 2016. Alternative approaches to vertebrate ecotoxicity tests in the 21st century: a reveiw of developments over the last two decades and current status. Env Tox Chem. 35: 2637-2646 http://onlinelibrary.wiley.com/doi/10.1002/etc.3603/epdf
  10. Bloch SR, Vo NTKWalsh SK, Chen CC, Lee LEJ, Hodson PV, Bols NC. 2016. Development of a cell line from the American eel brain expressing endothelial cell properties. In Vitro Cell Dev Biol Anim. 52(4): 395-409. http://link.springer.com/article/10.1007%2Fs11626-015-9986-8
  11. Lillicrap A, Belanger S, Burden N,  Embry M, Lee LEJ, Leonard M (2016) Is the Fish Embryo Acute Toxicity Test Under Threat? A Perspective from the SETAC Global Animal Alternatives Advisory Group. SETAC Globe 17(8) http://globe.setac.org/2016/august/fet-test.html
  12. Smith RW, Wang JX, Mothersill C, Lee LEJ, Seymour C. 2015. Proteomic responses in the gills of fathead minnows (Pimephales promelas, Rafinesque, 1820) after 6 months and 2 years of continuous exposure to environmentally relevant dietary 226Ra. Int J Radiation Biol. 91(3): 248-256. http://www.tandfonline.com/doi/abs/10.3109/09553002.2014.988894?journalCode=irab20
  13. Vo NTK, Chen C, Lee LEJ, Lumsden JS, Dixon B, Bols NC. 2015. Development and characterization of an endothelial cell line from the bulbus arteriosus of walleye, Sander vitreus. Comparative Biochem Physiol-A 180: 57-67. http://www.sciencedirect.com/science/article/pii/S1095643314002256
  14. Vo NTK, Bender AW, Lee LEJ, Lumsden JS, Lorenzen N, Dixon B, Bols NC. 2015. Development of a walleye cell line and use to study the effects of temperature on infection by viral hemorrhagic septicaemia virus (VHSV) group IVb. J Fis Dis 38(2): 121-136. http://onlinelibrary.wiley.com/doi/10.1111/jfd.12208/epdf
  15. Vo NTK, Mikhaeil MS, Lee LEJ, Pham PH, Bols NC. 2015. Senescence-associated β-galactosidase staining in fish cell lines and primary cultures from several tissues and species, including rainbow trout coelomic fluid and milt. In Vitro Cell Dev Biol Anim. 51(4): 361-371. http://link.springer.com/article/10.1007%2Fs11626-014-9837-z
  16. Gignac SJ, Vo NTK, Mikhaeil MS, Alexander JAN, MacLatchy DL, Schulte PM, Lee LEJ. 2014. Derivation of a continuous myogenic cell culture from an embryo of common killifish, Fundulus heteroclitus. Comparative Biochem Physiol A. 175:15-27.
  17. Vo NTK, Bufalino MR, Hartlen KD, Kitaev V, Lee LEJ. 2014. Cytotoxicity evaluation of silica nanoparticles using fish cell lines. In Vitro Cell Dev Biol–Anim. 50(5): 427-438.
  18. Sansom B, Vo NTK, Kavanagh R, Hanner R, MacKinnon M, Dixon DG, Lee LEJ. 2013. Rapid Assessment of the toxicity of oil sands process-affected waters using fish cell lines. In Vitro Cell. Dev. Biol.-Animal 49(1): 52-65.
  19. Scholz S, Sela E, Blaha L, Braunbeck T, Galay-Burgos M, García-Franco M, Guinea J, Klüver N, Schirmer K, Tanneberger K, Tobor-Kapłon M, Witters H, Belanger S, Benfenati E, Creton S, Cronin MTD, Eggen RIL, Embry M, Ekman D, Gourmelon A, Halder M, Hardy B, Hartung T, Hubesch B, Jungmann D, Lampi MA, Lee LEJ, Léonard M, Küster E, Lillicrap A, Luckenbach T, Murk AJ, Navas JM, Peijnenburg W, Repetto G, Salinas E, Schüürmann G, Spielmann H, Tollefsen KE, Walter-Rohde S, Whale G, Wheeler JR, Winter MJ. 2013. A European perspective on alternatives to animal testing for environmental hazard identification and risk assessment. Reg Tox Pharmacol 67(3): 506-530.
  20. Dayeh VR, Bols NC, Tanneberger K, Schirmer K, Lee LEJ. 2013. The use of fish derived cell lines for investigation of environmental contaminants – An Update following OECD’s fish toxicity testing framework n• 171. Current Protocols in Toxicology 56: 1.5.1–1.5.20.

Conference Presentations (last 2 years only)

  1. Braley E, Hay BN, Lee LEJ (2019) Evaluating the environmental risks of neonicotinoids in vitro: genotoxicity in fish cell lines. Canadian Ecotoxicity Workshop, Quebec City, QC. Oct 6-9
  2. Lee LEJ, Bols NC (2019) Development and uses of fish gill cell lines: an update with RTgill-W1 in toxicology. Canadian Ecotoxicity Workshop, Quebec City, QC. Oct 6-9
  3. Dowd GC, Chong G, Lee LEJ, Marshall S, Wellenreuther M (2019) Fish in vitro biology: another piece of the puzzle for the aquaculture of New Zealand native species Chrysophrys auratus. Society for In Vitro Biology meeting, Tampa, FL. June 8-13
  4. Hay BN, Lee LEJ (2019) Evaluating potential genotoxicity of imidacloprid with fish cell lines. Society for In Vitro Biology meeting, Tampa, FL. June 8-13
  5. Qanberry AM, Boyd TM, Webb TH, Lee LEJ (2019) Analyzing the Potential Effects of Temperature Changes on Fish Cell Lines. Soc. for In Vitro Biol. meeting, Tampa, FL. June 8-13
  6. Lee PWC, Miyagi AU, Opolko GC, Lee LEJ (2019) Edging closer to developing marine invertebrate cell lines. Society for In Vitro Biology meeting, Tampa, FL. June 8-13
  7. Bols NC, Pumputis PG, Dayeh VR, Lee LEJ (2019), 2016 Effects of the phytochemical, naringenin, on rainbow trout intestinal epithelial cells. Soc. for In Vitro Biology meeting, Tampa, FL. June 8-13
  8. Jenik K, Alkie TN, Moore E, Lee LEJ, DeWitte-Orr SJ (2019) Enhancing the efficacy of inosine monophosphate in production animals using a novel delivery system. Can Society of Zoologists annual meeting, Windsor, ON. May 13-16
  9. Lee LEJ (2019) In vitro fish meat in the making. Can Society of Zoologists annual meeting, Windsor, ON. May 13-16
  10. Hay BN, Lee LEJ (2019) Evaluating potential genotoxicity of imidacloprid with fish cell lines. Presented as a poster at both UFV Undergraduate Research Day. Mar 26, 2019, and at the 14th annual undergraduate research conference- UBC Okanagan. March 30, 2019
  11. Qanberry AM, Lee LEJ (2019) Analyzing the Potential Effects of Environmental Changes on Fish Cell Lines. Presented as a poster at the 14th annual undergraduate research conference- UBC Okanagan. March 30, 2019
  12. Boyd TM,Lee LEJ (2018) Long-Term In Vitro Culture of Olfactory Cells from Rainbow Trout (Oncorhynchus mykiss). Presented as a poster at UFV Undergraduate Research Day. Apr 5, 2018. ***Winner of UFV’s Student Research Day - President’s Award***
  13. Hay BN, Boyd TM, Sidhu HS, Moghrabi K, Oberoi V, Lee LEJ (2018) Evaluating potential cytotoxicity and genotoxicity of neonicotinoids with fish cell lines. 45th Canadian Ecotoxicity Workshop, Vancouver BC, Sep 30 - Oct 3, 2018, poster #23 Monday Oct 1. **Second Place Winner of Best Poster award at 45th CEW**
  14. Dayeh VR, Linton L, Hamilton M, Pham PH, Lee LEJ, Bols NC (2018) Evaluating the toxicity of beauvericin to rainbow trout intestinal epithelial cells at different temperatures. Annual Meeting of the Society for In Vitro Biology. St. Louis, MO. June 2-6, 2018, poster A2002
  15. Boyd TM, Gomez JM, Navarro SA, Lee LEJ (2018) Initiation, characterization and culture of olfactory cells from adult rainbow trout (Oncorhynchus mykiss). 57th Annual Meeting of the Canadian Society of Zoologists, Saint John’s, NL, May 7-11, 2018
  16. Webb TH, Boyd TM, Kobes VG, Navarro SA, Lee LEJ (2018) Establishment and characterization of mesothelial cell lines from rainbow trout (Oncorhynchus mykiss). 57th Annual Meeting of the Canadian Society of Zoologists, Saint John’s, NL, May 7-11, 2018
  17. Lee LEJ, Sidhu H, Kobes V, Pumputis P, Bols NC (2017) RTgutGC and RTS11-GFP Cell Lines as In Vitro Models to Study Fish Gut Physiology and Enteritis. Annual Meeting of the Society for In Vitro Biology. Raleigh, NC. June 10-14, 2017, poster A3014
  18. Sidhu H, Lee LEJ (2017) Trout enterocyte-macrophage co-culture system using RTgut-GC and RTS11-GFP cell lines as in vitro models to study gut physiology and enteritis. 56th Annual Meeting of the Canadian Society of Zoologists, Winnipeg, MB, May 15-19, 2017

 

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