Dr. Lee's research centers on the use of cell culture models derived from non-mammalian species, specifically fish and economically important invertebrates such as bees, shrimp, and lobsters.
Specifically, Dr. Lee's interest revolves around developing continuous cell lines that can be readily available and used for evaluating effects of nutrients, hormones, drugs, contaminants, toxicants, for applications in animal health, animal husbandry, biotechnology, environmental monitoring, etc.
Current projects funded by the Natural Sciences and Engineering Research Council of Canada (NSERC) include the development of fish cell lines and their use in the study of intracellular parasites like the microsporidia that have become emerging pathogens of concern.
Dr. Lee’s team members have used classical cell-culture methodologies along with proteomics and immunotechniques to understand interactions of host cells with model intracellular and extracellular aquatic pathogens. Recent interests have expanded to cover areas of concern in agriculture such as the study of microsporidians affecting bees, climate change and pesticide effects in non-target species, and evaluation of nutraceuticals, specifically, evaluating blueberry components for fish diets using fish intestinal cells (the latter funded by an NSERC Strategic).
Dr. Lee has adjunct faculty appointments at the University of British Columbia, Zoology Department; the University of Waterloo, Department of Biology; Wilfrid Laurier University, Department of Biology; as well as graduate appointments at the University of Guelph, Biomedical Sciences; and as such has supervised or co-supervised many graduate students.
At UFV, Dr. Lee has supervised several undergraduate and post-graduate students in various research projects and these include:
- Ryan Goldbach (2021) research assistant
- Heather Kelly (2020) volunteer assistant: general cell culture, quantitative methods, NSERC USRA (2021)
- Daylan Pritchard (2020) NSERC USRA
- Eryn Braley (2019): Evaluation of common pesticides on fish cell lines, NSERC USRA
- Eric Sung-Jae Cho (2019) research assistant: general cell culture, quantitative methods
- Brenna Hay (2019): Genotoxic effects of neonicotinoids in fish cells (NSERC USRA) **Pictured above
- Petra Lee (2019): Fish and shrimp cell culture
- Michael Mong (2019): Characterizing fish muscle cell lines (co-op UBC)
- Arianne Qanberry (2019): Effect of temperature fluctuations in vitro on fish mitochondria
- Anthony Miyagi (2018, 2019): Initiation and culture of invertebrate cells: bees and shrimp
- Taylor Boyd (2018): Culture of trout olfactory cells, NSERC-USRA
- Tessa Webb (2017-2018) research assistant: Fish mesenchymal cell culture maintenance and quality control
- Veronica Kobes (2017) research assistant: Histology of rainbow trout tissues, NSERC-USRA (2018)
- Sergio Navarro (2017): Primary cell cultures of rainbow trout, routine fish cell line growth
- Harshraj Sidhu (2016, 2017): In vitro toxicology; and Co-cultures of gastrointestinal and immune cells, NSERC USRARachael Hyun Bae (2016): Cell culture maintenance, mycoplasma testing
- Jose Martin Gomez (2016): UFV BSc Honours Thesis, visiting international student
- Gaganjeet Mahil (2016): Evaluation of blueberry extracts w fish cells, NSERC USRA
- Gagandeep Rai (2015-2016): Fish feed evaluation, NSERC USRA
- Manjot Birk (2015) volunteer: Routine fish cell line maintenance
- Kamal Moghrabi (2015): Neonicotinoid effects in fish cells, NSERC USRA
- Vishesh Oberoi (2015): Scratch assays, Blueberry extract effects on fish, NSERC USRA
- Hardeep Saran (2015) volunteer: Basic fish cell culture
- Alyssa Zucchet (2015): Cell maintenance, lab upkeep
- Kevin Kobes (2014): Evaluating physicochemical factors on the growth of fish and invertebrate cells, NSERC USRA
- Mandeep Kaur Saran (2014) Characterizing cod cells, NSERC USRA
- John Andrew Alexander (2013-2014): Quality control of fish and insect cells NSERC USRA
- Fabiola Rojas (2013-2014) technician: Assistance with cell culture lab maintenance and organization
- Marcus Cardoso Trento (2013): Evaluation of agricultural contaminants (Science without borders student from Brazil Genotoxic)